[Nhcoll-l] cleared and stained amphibians

[Bentley, Andrew Charles]

Tonya

We have sometimes mixed different proportions of glycerin and 70% ethanol to make the liquid less viscous and thus reduce bubble formation in cavities of C&S specimens but otherwise use full strength glycerin for these specimens with a couple of crystals of thymol added to prevent mold growth.  The addition of 70% ethanol, as long as not too much, does not seem to affect these specimens in any adverse way – that we can tell.

Andy

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Andy Bentley
Ichthyology Collection Manager
University of Kansas
Biodiversity Institute
Dyche Hall
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From: Nhcoll-l <nhcoll-l-bounces at mailman.yale.edu> on behalf of "Haff, Tonya (NCMI, Crace)" <Tonya.Haff at csiro.au>
Date: Wednesday, October 5, 2022 at 6:13 PM
To: "nhcoll-l at mailman.yale.edu" <nhcoll-l at mailman.yale.edu>
Subject: [Nhcoll-l] cleared and stained amphibians

Hello all,

We have some cleared and stained small frogs that are, I suspect, stored in a mixture of glycerine and ethanol. I have read in John’s Fluid Preservation book that some people use a mixture the two at a 1:1 ratio (but at what ETOH concentration I am not sure), but I wonder if any of you would share how you store similar specimens? Some of the jars need to be topped up, so I also wonder if it’s best to top up using a 1:1 ratio of glycerine to ethanol (given that’s what it *looks* like, ha ha), or remove all fluid and replace, or…? Thoughts, as usual, appreciated!

Cheers,

Tonya

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Dr. Tonya M. Haff
Collection Manager
Australian National Wildlife Collection
CSIRO
+61(0)419569109
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