[Nhcoll-l] Ethanol concentration for tissue samples

[Simon Moore]

Hi Sarah,


Presumably this relates to preservation of DNA?  If so, then it was agreed, a few years ago, that using 96% ethanol and stored at 5 deg. C was the best.  99% and absolute were found to be less effective.
I am sure there will be other comments relating to this.

With all good wishes, Simon.

Simon Moore MIScT, RSci, FLS, ACR
Conservator of Natural Sciences and Cutlery Historian,
www.natural-history-conservation.com 




On 27 Jan 2016, at 17:34, Sarah K. Huber <skhuber at vims.edu> wrote:

> I recently had a graduate student request 100% absolute ethanol to use for preserving tissue samples (fish muscle and/or fins). We routinely use 95% ethanol (and all tissue samples are then stored in a -80 freezer); however, the student is adamant that 100% is the only preservative that is acceptable for tissue preservation. Does anyone have an opinion on 95% versus 100% ethanol for tissue preservation? There is a very steep price difference between the two fluids, and my understanding is that 95% ethanol preserves tissues just as well as 100%.
>  
> Thanks,
> Sarah
> 
> Sarah K. Huber, Ph.D.
> Curatorial Associate, VIMS Nunnally Ichthyology Collection
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