[Nhcoll-l] FW: [CRUST-L:9947] Mercuric chloride and sequencing

[Paolo Viscardi]

Hi Rachael,

these are both quite old references and contemporary techniques can yield
usable data from much more fragmented DNA than was possible back in 2003. I
expect the High Throughput Sequencing techniques being used for formalin
treated material my be applicable to specimens preserved in mercuric
chloride:
https://urldefense.proofpoint.com/v2/url?u=http-3A__journals.plos.org_plosone_article-3Fid-3D10.1371_journal.pone.0141579&d=CwIFaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=7Hv0d9U59ARm-uJ3xwzPGUpdSBFvTyKM0ZHs1Gr8kUQ&s=wfyTfwZHLSNzoV9KY6fhmWfvpjFkXDgHSIRmQKVcMmA&e= 

Cheers,

Paolo

On 24 January 2017 at 20:14, Rachael Peart <rachaelapeart at gmail.com> wrote:

> Thanks for the discussion,
> It looks like due to it's toxic nature, there is no extraction possible
> from animals preserved with mercuric chloride based products.
> Found these articles.
> O'Leary et al, 1994 - "The importance of fixation procedures on DNA
> template and its suitability for solution-phase polymerase chain reaction
> and PCR *in situ *hybridisation."
> The Histochemical Journal 26 (4): 337 - 346.
> C. Schander and K.M. Halanych, 2003. "DNA, PCR and formalinized animal
> tissue - a short review and protocols." Organisms Diversity and Evolution 3
> (3): 195 - 205.
>
> Thanks anyway
>
> Rachael
>
> On Tue, Jan 24, 2017 at 9:50 PM, Joachim Haendel <joachim.haendel at zns.uni-
> halle.de> wrote:
>
>> Hello Kareen & Rachael,
>> and hello Dirk,
>>
>> well - this is really interesting. But I can't say anything about this.
>> To my knowledge there are no researches
>> on this topic. Mercury(II) chloride is an effective antiseptic and
>> disinfectant and it is corrosive. It is thinkable
>> that it will damage the DNA. Maybe someone could try it out?! I think
>> many museo-biologists want to know
>> the results.
>>
>> Greetings
>> Joachim
>>
>>
>>
>> ---- On 24 Jan 2017 at 8:36, Dirk Neumann wrote:
>>
>> > Good morning Kareen & Rachael,
>> >
>> > I am not sure if there is first hand experience how mercuric chloride
>> > behaves exactly, but it was used as fixative especially for marine
>> > invertebrates in the early 20th century (sublimate; cf. Piechocki &
>> > Händel, 2007. Makroskopische Präparationstechnik, Wirbellose, 5th
>> > Edition) and as early antibiotic (e.g. against syphilis) because of its
>> > toxic properties. So I would assume that it does interact with tissues
>> > and DNAs and consequently do influence sequencing results.
>> >
>> > Perhaps Joachim can add more details ?
>> >
>> > Hope this helps
>> > Dirk
>> >
>> > Am 23.01.2017 um 23:54 schrieb Kareen Schnabel:
>> > >
>> > > Good morning all, I thought this listserv may have some valuable input
>> > > into my colleague´s query to the Crustacean listserv. Please see query
>> > > below and please reply to Rachael Peart rachaelapeart at gmail.com
>> > > <mailto:rachaelapeart at gmail.com>.**
>> > >
>> > > Thanks
>> > >
>> > > Kareen
>> > >
>> > >
>> > > DrKareen Schnabel
>> > > Marine Biologist
>> > >
>> > > *From:*crust-l-request at vims.edu [mailto:crust-l-request at vims.edu] *On
>> > > Behalf Of *Rachael Peart
>> > > *Sent:* Tuesday, 24 January 2017 11:49 a.m.
>> > > *To:* crust-l at vims.edu
>> > > *Subject:* [CRUST-L:9947] Mercuric chloride and sequencing
>> > >
>> > > Hi all,
>> > >
>> > > I have come across some amphipod samples that were captured in traps
>> > > using mercuric chloride.
>> > >
>> > > I am curious to know if this chemical can affect molecular sequencing
>> > > - ie COI or 18S?
>> > >
>> > > Does it completely destroy the amounts, or do you get traces, or no
>> > > damage?
>> > >
>> > > Thanks in advance
>> > >
>> > > Rachael Peart
>> > >
>> > >
>>
>>
>>
>> --
>> Joachim Haendel
>> Center of Natural Sciences Collections
>> of the Martin-Luther-University
>> - Entomological Collection  -
>>
>> Domplatz 4
>> D-06099 Halle (Saale)
>> Germany
>>
>> Phone:  +49 345 - 55 26 447
>> Fax: +49 345 - 55 27 248
>> Email: joachim.haendel at zns.uni-halle.de
>>
>>
>>
>
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