[Nhcoll-l] FW: [CRUST-L:9947] Mercuric chloride and sequencing

[Dirk Neumann]

Good morning Kareen & Rachael,

I am not sure if there is first hand experience how mercuric chloride 
behaves exactly, but it was used as fixative especially for marine 
invertebrates in the early 20th century (sublimate; cf. Piechocki & 
Händel, 2007. Makroskopische Präparationstechnik, Wirbellose, 5th 
Edition) and as early antibiotic (e.g. against syphilis) because of its 
toxic properties. So I would assume that it does interact with tissues 
and DNAs and consequently do influence sequencing results.

Perhaps Joachim can add more details ?

Hope this helps
Dirk

Am 23.01.2017 um 23:54 schrieb Kareen Schnabel:
>
> Good morning all, I thought this listserv may have some valuable input 
> into my colleague’s query to the Crustacean listserv. Please see query 
> below and please reply to Rachael Peart rachaelapeart at gmail.com 
> <mailto:rachaelapeart at gmail.com>.**
>
> Thanks
>
> Kareen
>
>
> DrKareen Schnabel
> Marine Biologist
>
> +64-4-386-0862| 301 Evans Bay Parade, Greta Point, Wellington| 
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> *From:*crust-l-request at vims.edu [mailto:crust-l-request at vims.edu] *On 
> Behalf Of *Rachael Peart
> *Sent:* Tuesday, 24 January 2017 11:49 a.m.
> *To:* crust-l at vims.edu
> *Subject:* [CRUST-L:9947] Mercuric chloride and sequencing
>
> Hi all,
>
> I have come across some amphipod samples that were captured in traps 
> using mercuric chloride.
>
> I am curious to know if this chemical can affect molecular sequencing 
> - ie COI or 18S?
>
> Does it completely destroy the amounts, or do you get traces, or no 
> damage?
>
> Thanks in advance
>
> Rachael Peart
>
>
>
>
>
>
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-- 
Dirk Neumann

Tel: 089 / 8107-111
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