[Nhcoll-l] Phenoxyethanol and DNA

Fri Nov 12 08:14:23 EST 2021

Dirk: do you know (or does anyone else) the effect of Phenoxyethanol on DNA? If it does not degrade it, then we should be aware that refixing in formalin will and that snips should thus be taken ahead of that stage if future gene work is contemplated.

Paul Callomon
Collection Manager, Malacology and General Invertebrates
________________________________
Academy of Natural Sciences of Drexel University
1900 Benjamin Franklin Parkway, Philadelphia PA 19103-1195, USA
prc44 at drexel.edu<mailto:prc44 at drexel.edu> Tel 215-405-5096 - Fax 215-299-1170

From: Nhcoll-l <nhcoll-l-bounces at mailman.yale.edu> On Behalf Of Dirk Neumann
Sent: Friday, November 12, 2021 2:53 AM
To: nhcoll-l at mailman.yale.edu
Subject: Re: [Nhcoll-l] Phenoxyethanol

External.
Dear Tonya,

several recipes exist and have been used in collections since the mid 1950ies, mainly for marine invertebrates, but no only. Simon Moore published<https://nam10.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.natsca.org%2Fsites%2Fdefault%2Ffiles%2Fpublications%2Fbooks%2FFluid%2520Preservation.pdf&data=04%7C01%7Cprc44%40drexel.edu%7C939afca06c0f4ba50f7808d9a5b1b4fa%7C3664e6fa47bd45a696708c4f080f8ca6%7C0%7C1%7C637723004955162859%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=qOGYqNfaSmkouCEmUgptIcSpMtYrAfsHwXyVltDMx%2F0%3D&reserved=0> on it back in 1999 and colleagues from the NHM in London may chip-in.

John Simmons also has a own section on phenoxetol in his Fluid Preservation book<https://nam10.safelinks.protection.outlook.com/?url=https%3A%2F%2Frowman.com%2FISBN%2F9781442229662%2FFluid-Preservation-A-Comprehensive-Reference&data=04%7C01%7Cprc44%40drexel.edu%7C939afca06c0f4ba50f7808d9a5b1b4fa%7C3664e6fa47bd45a696708c4f080f8ca6%7C0%7C1%7C637723004955172856%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=HeuQSswtm30y5pq9UpmfuknnVezf9Z0gmg68WTRCIWo%3D&reserved=0> (pages 69-70)

The recommendation we give in our forthcoming book is that specimens from phenoxetol should first be re-fixed in a 4% formaldehyde solution for few days (depending on specimen size, body mass etc.) and then stepped up from 30-50-70% EtOH. This seems to have worked fine, but this experience so far refers exclusively to elasmobranchs, which tend to behave slightly different to fixatives and preservatives compared to, for example, bony fish (likely because of the cartilage). Close monitoring during stepping specimens up surely is advised.

Hope this helps
Dirk

Am 12.11.2021 um 01:43 schrieb Haff, Tonya (NCMI, Crace):
Hello all,

We just come upon some bird specimens in our collection that have been preserved with Phenoxyethanol.  There are no notes on its concentration. The fluid needs to be changed over. I think we should change it to 70% ETOH, but I am not sure if we should slowly step up the concentration, or if another solution would be preferable?

Thanks!

Tonya

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