[Nhcoll-l] Rite-in-Rain Paper
Dirk Neumann
dirk.neumann at zsm.mwn.de
Thu Jan 5 02:48:56 EST 2017
... apparently RIR is using
pure barium sulfate (insoluble in water and pH neutral) as paper
brightener (cf. wikipedia section); barium is a heavy metal and it might
be worth testing how much barium gets solved if immersed in 96% EthOH
https://urldefense.proofpoint.com/v2/url?u=https-3A__en.wikipedia.org_wiki_Barium-5Fsulfate&d=CwID-g&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=5JM_j-aYlqQ-PWhGjALnFjjsWtXgyTb3v9_aeeOwNMI&s=AjJo-zB3Snzj22ifaJoDVfeQygAgZjjX1bcIwuy118s&e=
Lucidene®605 seems to be not pH neutral (> 8.0), and might cause pH
shifts, especially in 96% EthOH which barely has any buffering
capacities, especially since RIR paper omits any calcium carbonate in
the paper
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.hydrite.com_Files_resources_Paints-2DCoatings-2DInks-2D-2DAdhesives_GraphicArtsIndustryBrochure-2Dv5.pdf&d=CwID-g&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=5JM_j-aYlqQ-PWhGjALnFjjsWtXgyTb3v9_aeeOwNMI&s=TE424emySUQKGDknSD6qQqYyZ3uyMO1dlCLz_00NxlM&e=
and one acrylic monomere (which they don't exactly want to disclose;
they are citing a broader range of such acrylates); these are small
acidic and highly reactive molecules, as the denaturant methyl ethyl
ketone (MEK) based on a propylene. It is the simplest unsaturated
carboxylic acid, and miscible in water, alcohols ethers and chloroform.
https://urldefense.proofpoint.com/v2/url?u=https-3A__en.wikipedia.org_wiki_Acrylic-5Facid&d=CwID-g&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=5JM_j-aYlqQ-PWhGjALnFjjsWtXgyTb3v9_aeeOwNMI&s=y7goomOq6zi4mkPu0unz-DoVq69dtQZm8Kelvt8B4vM&e=
Waterproof sheet od RIR are "/derived from monomers selected from the
group consisting of styrene, butyl acrylate, 2-ethylhexyl acrylate,
acrylic acid, and a mixture thereof/"
Acrylic acid reacts with alcohols and forms the corresponding esters;
respective esters and slats thereof are known as acrylates, which are
highly reactive; it also readily combine with themselves to form
polyarcylic acid or other suited monomers (containing ammonium) to
acrylonitrile or acrylamid.
It is not unlikely that it dissolves into the ethanol of the tissue
tubes and at least may cause pH shifts, but may also otherwise spoil the
DNA of included organisms.
If the overall pH remains neutral because of the Lucidene remains to be
tested, but from the ingredients that are disclosed, I would not use it
inside tubes, even though this might be handy in the field or
researchers love it the easy way.
Thanks for sharing the patent link, Dean - good to know that you can
look up patents online
All the best
Dirk
https://urldefense.proofpoint.com/v2/url?u=https-3A__www.google.com_patents_US6863940&d=CwID-g&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=5JM_j-aYlqQ-PWhGjALnFjjsWtXgyTb3v9_aeeOwNMI&s=PlKDI7uQye_UfoOE5FEGeYDID39iT0u39uvb9UccBak&e=
<https://urldefense.proofpoint.com/v2/url?u=https-3A__www.google.com_patents_US6863940&d=CwMFaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=8JYV-mWBX0lD8AixvlLRuftVR8_qnwyx93hXgAEgFYc&s=aEJw8PkEnLfzYtd_xIuDXppMv4Lemei4ZmcqUNAMOS4&e=>
Cheers!
-Dean
--
Dean Pentcheff
pentcheff at gmail.com <mailto:pentcheff at gmail.com>
Am 04.01.2017 um 22:39 schrieb Dirk Neumann:
> Hi Alexandra,
>
> I am not sure about the composition of RIR, but if uses formaldehyde
> e.g. in the pulp to glue it together, the paper might be reactive if
> it gets into contact with formalin, e.g. start to swell, get sticky /
> stick together. I once tried a high quality archival paper and put a
> small bundle of labels into formalin. As soon as they entered, they
> started to swell and stuck firmly together; it was impossible to
> detach individual labels from each other again.
>
> But there are also other chemicals that could do harm (e.g. shifting
> the pH). Normally, if a paper is some sort of water resistant, either
> the surface and bottom and/or the pulp is hydrophobised. Normal paper
> already uses up to 30 chemicals, waterproofed paper surely more. Even
> if you use RIR only temporarily in the field until samples are
> returned, you may already have a chemical cocktail inside because 96%
> EthOH is a fairly good organic solvent, too.
>
> For me, externally pre-numbered tubes seem the safer option; or tubes
> that can be marked externally with pencils. If those internally RIR
> tagged samples fail during extraction or give bad sequencing results,
> you add just one more unknown to the black box of weird lab mysteries
> making samples fail.
>
> I guess the only other option would be to specifically test for
> effects of internal RIR tags by subdividing a tissue to two tubes, one
> externally labelled, the other with internal RIR, returned under
> identical conditions to the lab, and compare e.g. DNA yields after
> extraction, PCR results, etc. if you want to be sure. For sure still
> not the answer you are looking for, but hope it offers some sort of help.
>
> All the best
> Dirk
>
>
> Am 04.01.2017 um 21:20 schrieb Alexandra Snyder:
>> Andy, Absolutely. No disagreement there, i.e., external labels.
>> Thanks for reminding the list.
>>
>> I would still like to document the condition of tissue accessions
>> rec'd with RIR labels and the USFWS hatchery folks are curious re:
>> the use of Rite in Rain for temporary labels.
>>
>> If there is a way to get an idea or if anyone has knowledge of RIR
>> properties, I would like to hear about it. I recognize that
>> manufacturers do not share recipes, but one never knows.
>>
>> Lex
>>
>> On Wed, Jan 4, 2017 at 11:41 AM, Bentley, Andrew Charles
>> <abentley at ku.edu <mailto:abentley at ku.edu>> wrote:
>>
>> Lex
>>
>> We have a policy here at KU that nothing gets put inside the tube
>> except the tissue and preservative (if necessary). There are
>> just too many possible avenues of contamination from the “paper”
>> to the “ink” to the residue left on the paper by human contact.
>> All labels are external and usually adhered before going in to
>> the field using a Brady labeling system and cryo safe labeling
>> material.
>>
>> Andy
>>
>> A : A : A :
>>
>> }<(((_°>.,.,.,.}<(((_°>.,.,.,.}<)))_°>
>>
>> V V V
>>
>> Andy Bentley
>>
>> Ichthyology Collection Manager
>>
>> University of Kansas
>>
>> Biodiversity Institute
>>
>> Dyche Hall
>>
>> 1345 Jayhawk Boulevard
>>
>> Lawrence, KS, 66045-7561
>>
>> USA
>>
>> Tel: (785) 864-3863 <tel:%28785%29%20864-3863>
>>
>> Fax: (785) 864-5335 <tel:%28785%29%20864-5335>
>>
>> Email: abentley at ku.edu <mailto:abentley at ku.edu>
>>
>> https://urldefense.proofpoint.com/v2/url?u=http-3A__ichthyology.biodiversity.ku.edu&d=CwID-g&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=5JM_j-aYlqQ-PWhGjALnFjjsWtXgyTb3v9_aeeOwNMI&s=RAcTAofSOfx9zhHD7KmGhEIb2bKFgRPVRtM7NfFMick&e=
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>>
>> SPNHC President
>>
>> https://urldefense.proofpoint.com/v2/url?u=http-3A__www.spnhc.org&d=CwID-g&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=5JM_j-aYlqQ-PWhGjALnFjjsWtXgyTb3v9_aeeOwNMI&s=pxlFfLGuovGbyKxUf0cJ-pPkubimVryX5jQeNQva_y0&e=
>> <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.spnhc.org_&d=CwMFaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=yeVZknhKpfJqjG6xkBiY19DaPybnQNUVHw1E7RGzJs4&s=Gd08bi3F872H0ud78Rdp2-UUcw3r1viukH0F890Zioc&e=>
>>
>> : :
>>
>> A : A : A :
>>
>> }<(((_°>.,.,.,.}<(((_°>.,.,.,.}<)))_°>
>>
>> V V V
>>
>> *From: *<nhcoll-l-bounces at mailman.yale.edu
>> <mailto:nhcoll-l-bounces at mailman.yale.edu>> on behalf of
>> Alexandra Snyder <amsnyder at unm.edu <mailto:amsnyder at unm.edu>>
>> *Date: *Wednesday, January 4, 2017 at 12:10 PM
>> *To: *"nhcoll-l at mailman.yale.edu
>> <mailto:nhcoll-l at mailman.yale.edu>" <nhcoll-l at mailman.yale.edu
>> <mailto:nhcoll-l at mailman.yale.edu>>
>> *Subject: *[Nhcoll-l] Rite-in-Rain Paper
>>
>> Rite-in-Rain or "all weather" field paper is used by the many
>> agency biologists depositing specimens and genetic samples with
>> the MSB Division of Fishes and other repositories. Recently,
>> the USFWS Southwestern Native Aquatic Resources and Recovery
>> Center has requested that I find out if there are any issues with
>> using this paper for genetic labels in the field or otherwise.
>> They tried to get information from the Rite-in-Rain company re:
>> what was in the paper and coating (formalin?) to determine if the
>> paper would pose a problem for genetic samples. The company was
>> not forthcoming re: Rite-in-Rain product specifics.
>>
>> We have had many genetic samples received at both the Center and
>> the MSB with these paper tags made by tearing pieces of paper
>> out of the RIR field books, writing the sample number on RIR tag
>> with pencil, and stuffing the tag into a cryotube. Not optimal
>> in terms of contamination issues, etc. but getting word out to
>> field crews, etc. can be difficult. For many projects,
>> I preprint labels on polyspun paper (DataMax) for
>> researchers, but there are many opportunistic collections that
>> receive these temporary labels.
>>
>> Any thoughts or insights? Does anyone have an idea of how this
>> paper is manufactured/treated?
>>
>> Thank you.
>>
>> *************************************
>>
>> Alexandra M Snyder, Collections Manager-Fishes
>>
>> Museum of Southwestern Biology MSC03-2020
>>
>> University of New Mexico Albuquerque NM 87131
>>
>> PH./FAX 505.277.6005 <tel:%28505%29%20277-6005> amsnyder at unm.edu
>> <mailto:amsnyder at unm.edu>
>>
>> PHYSICAL ADDRESS FOR FEDEX/UPS
>>
>> Bldg.83 Room 204
>>
>> 302 Yale Blvd NE
>>
>>
>>
>>
>> --
>> *****************************************************
>> Alexandra M Snyder
>> Collections Manager-Fishes
>> Museum of Southwestern Biology MSC01-2020
>> University of New Mexico
>> Albuquerque NM 87131 USA
>>
>> PH.505.277.6005 OFFICE
>>
>> _Physical address for FedEx and UPS_
>> Bldg.83 Room 204, 302 Yale Blvd NE
>>
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>>
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> --
> Dirk Neumann
>
> Tel: 089 / 8107-111
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> ---------
>
> Dirk Neumann
>
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Tel: 089 / 8107-111
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email: Dirk.Neumann(a)zsm.mwn.de
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