[Nhcoll-l] frozen tissue collections
Bentley, Andrew Charles
abentley at ku.edu
Mon Aug 16 15:06:22 EDT 2021
Catherine
No, I would not say that is the case. It very much depends on how these have been treated over the years as to whether viable DNA can be extracted from them. DNA has been extracted from worse. If they have been frozen at -20 consistently since collecting then there is every possibility that viable DNA may still be present. If they have all been treated the same way it may be useful to test a couple of them to see if you can get DNA out of them and then progress from there.
Andy
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Andy Bentley
Ichthyology Collection Manager
University of Kansas
Biodiversity Institute
Dyche Hall
1345 Jayhawk Boulevard
Lawrence, KS, 66045-7561
USA
Tel: (785) 864-3863
Fax: (785) 864-5335
Email: abentley at ku.edu<mailto:abentley at ku.edu>
http://ichthyology.biodiversity.ku.edu<http://ichthyology.biodiversity.ku.edu/>
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From: Nhcoll-l <nhcoll-l-bounces at mailman.yale.edu> on behalf of "Catherine Early (she/her)" <cearly at smm.org>
Date: Monday, August 16, 2021 at 1:47 PM
To: "nhcoll-l at mailman.yale.edu" <nhcoll-l at mailman.yale.edu>
Subject: Re: [Nhcoll-l] frozen tissue collections
Hi all,
I've dug into some of the very helpful resources you shared and would appreciate some further input. At this point, we are focusing our efforts on working through a 20 year backlog of carcasses stored in -20C freezers awaiting more traditional specimen preps, rather than collecting new specimens. Most of these specimens are relatively common species that were salvaged or were put into coolers during field surveys and then frozen. I thought it would be good to also preserve tissues for genomic studies as we prepare these specimens, but after reading the papers you shared, it seems like these specimens have little to no chance of yielding useful genetic material given their storage history so we shouldn't bother preserving tissue samples from them. Is that correct?
Best,
Catherine
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Catherine M. Early, PhD
she/her
Barbara Brown Chair of Ornithology
e: cearly at smm.org<mailto:cearly at smm.org>
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We envision a world where everyone has the power to use science to make lives better,
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On Fri, Aug 13, 2021 at 11:47 AM Catherine Early (she/her) <cearly at smm.org<mailto:cearly at smm.org>> wrote:
Thanks to everyone who replied with resources! It's great to be part of a community of people so eager to help.
Best,
Catherine
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Catherine M. Early, PhD
she/her
Barbara Brown Chair of Ornithology
e: cearly at smm.org<mailto:cearly at smm.org>
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We envision a world where everyone has the power to use science to make lives better,
and we are committed to using STEM as a tool to advocate for justice and equity.
On Thu, Aug 12, 2021 at 8:38 AM Catherine Early (she/her) <cearly at smm.org<mailto:cearly at smm.org>> wrote:
Hi all,
We're going to start taking tissue samples for genomic work as part of our specimen preparations. I'm currently writing a grant to upgrade our collections and will include a request for a tissue sample freezer, but I'm really struggling to write that section since I don't use tissues in my own research. Can you recommend good books, papers, or other resources to get me started? Thanks!
Best,
Catherine
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Catherine M. Early, PhD
she/her
Barbara Brown Chair of Ornithology
e: cearly at smm.org<mailto:cearly at smm.org>
https://catherineearly.wixsite.com/home<https://nam10.safelinks.protection.outlook.com/?url=https%3A%2F%2Fcatherineearly.wixsite.com%2Fhome&data=04%7C01%7Cabentley%40ku.edu%7C69c2cd733a184a78137408d960e62aeb%7C3c176536afe643f5b96636feabbe3c1a%7C0%7C0%7C637647364689659064%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=A0N6SeiayEJPrxQ0roCOzgCkX%2BExMaXDml86TVzmOaw%3D&reserved=0>
We envision a world where everyone has the power to use science to make lives better,
and we are committed to using STEM as a tool to advocate for justice and equity.
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