[Nhcoll-l] frozen tissue collections

[Catherine Early (she/her)]

Hi Andy,

They haven't all been treated the same way and there is little if any
record-keeping on how they were treated, especially the salvage specimens
brought to us by members of the public. But the feedback I'm getting is
that it would still be worth taking tissue samples, and worth writing a
small grant to cover some test extractions to see how they do. Thanks!

Best,
Catherine

<https://www.smm.org/>

*Catherine M. Early, PhD*

she/her

*Barbara Brown Chair of Ornithology*

e: cearly at smm.org

https://catherineearly.wixsite.com/home

We envision a world where everyone has the power to use science to make
lives better,
and we are committed to using STEM as a tool to advocate for justice and
equity.


On Mon, Aug 16, 2021 at 2:06 PM Bentley, Andrew Charles <abentley at ku.edu>
wrote:

> Catherine
>
>
>
> No, I would not say that is the case.  It very much depends on how these
> have been treated over the years as to whether viable DNA can be extracted
> from them.  DNA has been extracted from worse.  If they have been frozen at
> -20 consistently since collecting then there is every possibility that
> viable DNA may still be present.  If they have all been treated the same
> way it may be useful to test a couple of them to see if you can get DNA out
> of them and then progress from there.
>
>
>
> Andy
>
>
>
>      A  :                A  :               A  :
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>  }<(((_°>.,.,.,.}<(((_°>.,.,.,.}<)))_°>
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>      V                   V                  V
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> Andy Bentley
>
> Ichthyology Collection Manager
>
> University of Kansas
>
> Biodiversity Institute
>
> Dyche Hall
>
> 1345 Jayhawk Boulevard
>
> Lawrence, KS, 66045-7561
>
> USA
>
>
>
> Tel: (785) 864-3863
>
> Fax: (785) 864-5335
>
> Email: abentley at ku.edu
>
> http://ichthyology.biodiversity.ku.edu
>
>
>
>      A  :                A  :                A  :
>
>  }<(((_°>.,.,.,.}<(((_°>.,.,.,.}<)))_°>
>
>      V                   V                   V
>
>
>
>
>
> *From: *Nhcoll-l <nhcoll-l-bounces at mailman.yale.edu> on behalf of
> "Catherine Early (she/her)" <cearly at smm.org>
> *Date: *Monday, August 16, 2021 at 1:47 PM
> *To: *"nhcoll-l at mailman.yale.edu" <nhcoll-l at mailman.yale.edu>
> *Subject: *Re: [Nhcoll-l] frozen tissue collections
>
>
>
> Hi all,
>
>
>
> I've dug into some of the very helpful resources you shared and would
> appreciate some further input. At this point, we are focusing our efforts
> on working through a 20 year backlog of carcasses stored in -20C freezers
> awaiting more traditional specimen preps, rather than collecting new
> specimens. Most of these specimens are relatively common species that were
> salvaged or were put into coolers during field surveys and then frozen. I
> thought it would be good to also preserve tissues for genomic studies as we
> prepare these specimens, but after reading the papers you shared, it seems
> like these specimens have little to no chance of yielding useful genetic
> material given their storage history so we shouldn't bother preserving
> tissue samples from them. Is that correct?
>
>
>
> Best,
>
> Catherine
>
>
>
>
> <https://nam10.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.smm.org%2F&data=04%7C01%7Cabentley%40ku.edu%7C69c2cd733a184a78137408d960e62aeb%7C3c176536afe643f5b96636feabbe3c1a%7C0%7C0%7C637647364689629105%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=44O2PZenCzqtsYNB60aYnwRvi8jtILwOW8vi5Q1JhiQ%3D&reserved=0>
>
> *Catherine M. Early, PhD*
>
> she/her
>
> *Barbara Brown Chair of Ornithology*
>
> e: cearly at smm.org
>
> https://catherineearly.wixsite.com/home
> <https://nam10.safelinks.protection.outlook.com/?url=https%3A%2F%2Fcatherineearly.wixsite.com%2Fhome&data=04%7C01%7Cabentley%40ku.edu%7C69c2cd733a184a78137408d960e62aeb%7C3c176536afe643f5b96636feabbe3c1a%7C0%7C0%7C637647364689639074%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=2BoCFrfzn%2BdAnricSzYDv9C1BavCICFM162dQccvmKY%3D&reserved=0>
>
>
>
> We envision a world where everyone has the power to use science to make
> lives better,
> and we are committed to using STEM as a tool to advocate for justice and
> equity.
>
>
>
>
>
> On Fri, Aug 13, 2021 at 11:47 AM Catherine Early (she/her) <cearly at smm.org>
> wrote:
>
> Thanks to everyone who replied with resources! It's great to be part of a
> community of people so eager to help.
>
>
>
> Best,
>
> Catherine
>
>
>
>
> <https://nam10.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.smm.org%2F&data=04%7C01%7Cabentley%40ku.edu%7C69c2cd733a184a78137408d960e62aeb%7C3c176536afe643f5b96636feabbe3c1a%7C0%7C0%7C637647364689639074%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=fEOxsV8w%2F%2BpX2ef67o3B8HDBs273XLEg%2FWJJGwFhW%2FQ%3D&reserved=0>
>
> *Catherine M. Early, PhD*
>
> she/her
>
> *Barbara Brown Chair of Ornithology*
>
> e: cearly at smm.org
>
> https://catherineearly.wixsite.com/home
> <https://nam10.safelinks.protection.outlook.com/?url=https%3A%2F%2Fcatherineearly.wixsite.com%2Fhome&data=04%7C01%7Cabentley%40ku.edu%7C69c2cd733a184a78137408d960e62aeb%7C3c176536afe643f5b96636feabbe3c1a%7C0%7C0%7C637647364689649072%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=PZL1oAuc5BqlonRd28krbO9ebCgjhv5NKSv0Cm7oMtA%3D&reserved=0>
>
>
>
> We envision a world where everyone has the power to use science to make
> lives better,
> and we are committed to using STEM as a tool to advocate for justice and
> equity.
>
>
>
>
>
> On Thu, Aug 12, 2021 at 8:38 AM Catherine Early (she/her) <cearly at smm.org>
> wrote:
>
> Hi all,
>
>
>
> We're going to start taking tissue samples for genomic work as part of our
> specimen preparations. I'm currently writing a grant to upgrade our
> collections and will include a request for a tissue sample freezer, but I'm
> really struggling to write that section since I don't use tissues in my own
> research. Can you recommend good books, papers, or other resources to get
> me started? Thanks!
>
>
>
> Best,
>
> Catherine
>
>
>
>
> <https://nam10.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.smm.org%2F&data=04%7C01%7Cabentley%40ku.edu%7C69c2cd733a184a78137408d960e62aeb%7C3c176536afe643f5b96636feabbe3c1a%7C0%7C0%7C637647364689649072%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=JDbzkwFb%2BHlKReDjTpR5JYCzD%2BOlyyiQQxc7b0bKDDY%3D&reserved=0>
>
> *Catherine M. Early, PhD*
>
> she/her
>
> *Barbara Brown Chair of Ornithology*
>
> e: cearly at smm.org
>
> https://catherineearly.wixsite.com/home
> <https://nam10.safelinks.protection.outlook.com/?url=https%3A%2F%2Fcatherineearly.wixsite.com%2Fhome&data=04%7C01%7Cabentley%40ku.edu%7C69c2cd733a184a78137408d960e62aeb%7C3c176536afe643f5b96636feabbe3c1a%7C0%7C0%7C637647364689659064%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=A0N6SeiayEJPrxQ0roCOzgCkX%2BExMaXDml86TVzmOaw%3D&reserved=0>
>
>
>
> We envision a world where everyone has the power to use science to make
> lives better,
> and we are committed to using STEM as a tool to advocate for justice and
> equity.
>
>
>
>
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