[Nhcoll-l] collecting and preserving animal tissue samples

[Lennart Lennuk]

Thank you all for good material and advice!
I have one more question:
How much blood is considered enough for DNA sample?

Best!
Lennart

From: Emily M. Braker [mailto:emily.braker at colorado.edu]
Sent: Thursday, September 30, 2021 4:46 PM
To: Lennart Lennuk <Lennart.Lennuk at loodusmuuseum.ee>; nhcoll-l at mailman.yale.edu
Subject: Re: collecting and preserving animal tissue samples

Greetings Lennart,

I recommend taking a look at the SPNHC wiki for guidance on collecting and storing tissue samples:

https://spnhc.biowikifarm.net/wiki/Tissue_Sample_Collection
https://spnhc.biowikifarm.net/wiki/Genetic_Resources

But in short, we use 2ml externally threaded cryovials for tissue samples (many brands available from lab suppliers). Using 95% ETOH is an effective preservative and can be stored in a regular freezer if you don't have access to ultracold freezing or liquid nitrogen. Volume of tissue harvested is up to your institution based on anticipated usage, space, and species rarity. We tend to take three vials (heart, muscle, liver), but many institutions combine these samples into one vial, while others may sample additional organs. If we receive a rare or endangered species, we tend to increase our sampling.

Best,
Emily

Emily Braker
Vertebrate Collections Manager, Zoology Section
University of Colorado Museum of Natural History
265 UCB, Bruce Curtis Building
Boulder, CO 80309-0218
Phone: 303-492-8466
http://www.colorado.edu/cumuseum/research-collections/vertebrates

________________________________
From: Nhcoll-l <nhcoll-l-bounces at mailman.yale.edu<mailto:nhcoll-l-bounces at mailman.yale.edu>> on behalf of Lennart Lennuk <Lennart.Lennuk at loodusmuuseum.ee<mailto:Lennart.Lennuk at loodusmuuseum.ee>>
Sent: Thursday, September 30, 2021 5:36 AM
To: nhcoll-l at mailman.yale.edu<mailto:nhcoll-l at mailman.yale.edu> <nhcoll-l at mailman.yale.edu<mailto:nhcoll-l at mailman.yale.edu>>
Subject: [Nhcoll-l] collecting and preserving animal tissue samples


Dear all,



Do you have good material about collecting and preserving animal tissues?

I already search older nh-coll list letters and spnhc wiki.



I have some specific questions:

-          what vials are best for preserving the tissue samples?

-          does 96 EtOH in -25 Celsius work? No money for -80 freezer

-          How many tissues per animal is optimal?



Our purose will be to preserve the tissue samples of the animals we make for taxidermy collections in case a researcher

wants to have a DNA sample of the animal.



Best regards!

Lennart Lennuk

Head of collections

Estonian Museum of Natural History

+372 6603404, 56569916


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