[Nhcoll-l] collecting and preserving animal tissue samples

[Elizabeth Wommack]

Hello Lennart,

That would probably depend on what type of genetic/genomic analysis you
plan on conducting with it, as well as the taxon you are working with. You
may also want to think about the IACUC protocols for the species, as blood
draw amounts can be dictated by size of the animal and experimental
protocol.

Birds have nucleated blood cells, so I have gotten DNA extractions from
very small draws of blood.

cheers,
Beth Wommack

On Thu, Oct 14, 2021 at 3:07 AM Lennart Lennuk <
Lennart.Lennuk at loodusmuuseum.ee> wrote:

> ◆ This message was sent from a non-UWYO address. Please exercise caution
> when clicking links or opening attachments from external sources.
>
> Thank you all for good material and advice!
>
> I have one more question:
>
> How much blood is considered enough for DNA sample?
>
>
>
> Best!
>
> Lennart
>
>
>
> *From:* Emily M. Braker [mailto:emily.braker at colorado.edu]
> *Sent:* Thursday, September 30, 2021 4:46 PM
> *To:* Lennart Lennuk <Lennart.Lennuk at loodusmuuseum.ee>;
> nhcoll-l at mailman.yale.edu
> *Subject:* Re: collecting and preserving animal tissue samples
>
>
>
> Greetings Lennart,
>
>
>
> I recommend taking a look at the SPNHC wiki for guidance on collecting and
> storing tissue samples:
>
>
>
> https://spnhc.biowikifarm.net/wiki/Tissue_Sample_Collection
>
> https://spnhc.biowikifarm.net/wiki/Genetic_Resources
>
>
>
> But in short, we use 2ml externally threaded cryovials for tissue samples
> (many brands available from lab suppliers). Using 95% ETOH is an effective
> preservative and can be stored in a regular freezer if you don't have
> access to ultracold freezing or liquid nitrogen. Volume of tissue harvested
> is up to your institution based on anticipated usage, space, and species
> rarity. We tend to take three vials (heart, muscle, liver), but many
> institutions combine these samples into one vial, while others may sample
> additional organs. If we receive a rare or endangered species, we tend to
> increase our sampling.
>
>
>
> Best,
> Emily
>
>
>
> Emily Braker
>
> Vertebrate Collections Manager, Zoology Section
>
> University of Colorado Museum of Natural History
>
> 265 UCB, Bruce Curtis Building
>
> Boulder, CO 80309-0218
>
> Phone: 303-492-8466
>
> http://www.colorado.edu/cumuseum/research-collections/vertebrates
>
>
> ------------------------------
>
> *From:* Nhcoll-l <nhcoll-l-bounces at mailman.yale.edu> on behalf of Lennart
> Lennuk <Lennart.Lennuk at loodusmuuseum.ee>
> *Sent:* Thursday, September 30, 2021 5:36 AM
> *To:* nhcoll-l at mailman.yale.edu <nhcoll-l at mailman.yale.edu>
> *Subject:* [Nhcoll-l] collecting and preserving animal tissue samples
>
>
>
> Dear all,
>
>
>
> Do you have good material about collecting and preserving animal tissues?
>
> I already search older nh-coll list letters and spnhc wiki.
>
>
>
> I have some specific questions:
>
> -          what vials are best for preserving the tissue samples?
>
> -          does 96 EtOH in -25 Celsius work? No money for -80 freezer
>
> -          How many tissues per animal is optimal?
>
>
>
> Our purose will be to preserve the tissue samples of the animals we make
> for taxidermy collections in case a researcher
>
> wants to have a DNA sample of the animal.
>
>
>
> Best regards!
>
> Lennart Lennuk
>
> Head of collections
>
> Estonian Museum of Natural History
>
> +372 6603404, 56569916
>
>
>


-- 
Elizabeth Wommack, PhD
Curator and Collections Manager of Vertebrates
University of Wyoming Museum of Vertebrates
Berry Biodiversity Conservation Center
University of Wyoming,
Laramie, WY 82071
ewommack@ <ewommack at berkeley.edu>uwyo.edu
www.uwymv. <http://www.uwymv.edu/>org
UWYMV Collection Use Policy
<http://www.uwymv.org/index.php/download_file/view/43/143/>
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