[Nhcoll-l] DICE-CT on NHspecimens

[David Cannatella]

Hi all,
I thought I'd share some non-experimental observations on Lugol's solution
and its effect on formalin fixed material, specifically frogs.

I have not used in DICE-CT. However, I have dissected muscles on hundreds
of frogs, using Lugol's to improve visualization of muscles and enhance
photography.

I used a "standard" histology-type solution of iodine and potassium iodide
in 70% ethanol, not water. I'd reflect the skin to allow the stain to
penetrate quickly, and did the dissections in layers, exposing deeper
muscles and re-staining. I moved the specimen directly for storage alcohol
to the Lugols with no rinsing. Often a specimen was re-stained
several times, and destained in 70% ethanol (which was discarded after a
few uses). I never noticed any desiccation or softening of the muscles or
other organs such as the liver or intestines, but I was not collecting data
on this.
I did not measure changes in pH, but I threw out the Lugols after a week
to avoid potential acidification. I did not notice softening of bones, as
some have seen.

After muscle dissections, I would sometimes clear and stain the specimen
using standard alizarin-alcian blue techniques. The specimens seemed to
take up both the alizarin and alcian stains more quickly and evenly than
did an untreated specimen.  Perhaps this was a mordant effect of the iodine.

Based on these qualitative but numerous observations, as a curator I would
have little reservation about allowing DICE-CT staining of specimens, but
bear in mind that I was working with generally smaller vertebrates that
were sufficiently stained in 3-6 hours.

Of course, it should be noted in the catalog that the specimen was treated
in this way, to inform future researchers.

Best,
Dave








On Wed, Mar 23, 2022 at 9:01 AM Bentley, Andrew Charles <abentley at ku.edu>
wrote:

> Esther
>
>
>
> The oVert project here in the US has had extensive experience with diceCT
> and have developed the attached protocol.  Jaimi Gray would be the person
> to contact in this regard - jaimigray at floridamuseum.ufl.edu.  She also
> co-authored the following publication -
> https://onlinelibrary.wiley.com/doi/full/10.1002/ece3.7467
>
>
>
> Hope that helps
>
>
>
> Andy
>
>     A  :             A  :             A  :
>  }<(((_°>.,.,.,.}<(((_°>.,.,.,.}<)))_°>
>     V                V                V
> Andy Bentley
> Ichthyology Collection Manager
> University of Kansas
> Biodiversity Institute
>
> Dyche Hall
> 1345 Jayhawk Boulevard
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> USA
>
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>
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>
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>
>
> *From:* Nhcoll-l <nhcoll-l-bounces at mailman.yale.edu> * On Behalf Of *Esther
> Dondorp
> *Sent:* Wednesday, March 23, 2022 4:24 AM
> *To:* nhcoll-l at mailman.yale.edu
> *Subject:* [Nhcoll-l] DICE-CT on NHspecimens
>
>
>
> Dear colleagues,
>
>
>
> Does anyone have experience with Diffusible Iodine-based
> Contrast-enhanced Computed Tomography (DICE-CT) performed on fluid
> preserved natural history specimens? I want to know if it is really non
> destructive, if the specimens do stay stable for the next many years or
> more after this treatment?
>
>
>
> Many thanks!
>
>
>
> Esther Dondorp
>
> Senior Collectiebeheerder
>
>
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-- 
David Cannatella
Assoc. Director, Biodiversity Center
Department of Integrative Biology
Patterson Labs, 2415 Speedway
University of Texas, Austin, Texas 78712
www.cannatellalab.org
512.453.1620 cell
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